Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 421
... zone electrophoretic patterns as moving - boundary patterns . In particular , cognizance must be taken of the fact that multiple zones need not necessarily indicate inherent heterogeneity . As will be shown below , a single ...
... zone electrophoretic patterns as moving - boundary patterns . In particular , cognizance must be taken of the fact that multiple zones need not necessarily indicate inherent heterogeneity . As will be shown below , a single ...
Page 425
Sydney J. Leach. + b 60 the initial zone is taken as the distance b in Fig . 21a . If no field is applied , the initial narrow zone will spread with time , owing to diffusion . During electrophoresis , however , diffusion is modified by ...
Sydney J. Leach. + b 60 the initial zone is taken as the distance b in Fig . 21a . If no field is applied , the initial narrow zone will spread with time , owing to diffusion . During electrophoresis , however , diffusion is modified by ...
Page 436
... zone patterns are presented in Figs . 26a and 26b ; whereas the protein gave a single zone in phosphate buffer , two zones were obtained in phosphate - borate , the faster - moving zone being the major one . In order to determine ...
... zone patterns are presented in Figs . 26a and 26b ; whereas the protein gave a single zone in phosphate buffer , two zones were obtained in phosphate - borate , the faster - moving zone being the major one . In order to determine ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Dielectric Properties of Proteins 7 | 7 |
The Enhancement of Contrast | 21 |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone