Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 339
... RNase at 30 ° C indicated greater structural unfolding of the peptide chain than the spec- trum of thermally denatured RNase . Both the aliphatic methyl protons and the aromatic region showed much more pronounced sharpening than on ...
... RNase at 30 ° C indicated greater structural unfolding of the peptide chain than the spec- trum of thermally denatured RNase . Both the aliphatic methyl protons and the aromatic region showed much more pronounced sharpening than on ...
Page 343
... RNase S ' when compared with RNase S allowed the missing His - 12 proton to be assigned to peak 2 and therefore His - 112 to peak 3. The anomalous chemical shift in the acid pH region of peak 4 ( Fig . 33 ) , and its broad line width in ...
... RNase S ' when compared with RNase S allowed the missing His - 12 proton to be assigned to peak 2 and therefore His - 112 to peak 3. The anomalous chemical shift in the acid pH region of peak 4 ( Fig . 33 ) , and its broad line width in ...
Page 406
... RNase - 3 ' - CMP complex . Concentrations are RNase , 0.0065 M ; 3 ' - CMP , 0.03 M. Above pH 7 the enzyme is no longer saturated with inhibitor . Dashed lines show titration curves of His peaks in RNase alone . .. RNase A ; RNase A + ...
... RNase - 3 ' - CMP complex . Concentrations are RNase , 0.0065 M ; 3 ' - CMP , 0.03 M. Above pH 7 the enzyme is no longer saturated with inhibitor . Dashed lines show titration curves of His peaks in RNase alone . .. RNase A ; RNase A + ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone