Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 127
... acid and stronger organic acids . Thus the polyelectrolyte effect has been observed for poly- amides dissolved in formic acid ( Schaefgen and Trivisonno , 1951 , 1952 ; Saunders , 1962 , 1964 ) , poly - L - amino acids in mixtures of ...
... acid and stronger organic acids . Thus the polyelectrolyte effect has been observed for poly- amides dissolved in formic acid ( Schaefgen and Trivisonno , 1951 , 1952 ; Saunders , 1962 , 1964 ) , poly - L - amino acids in mixtures of ...
Page 139
... acid or trifluoroacetic acid to the helicogenic solvent . This is shown by the fact that PBLG in 80 % ethylene dichloride- 20 % dichloroacetic acid gives a polyelectrolyte effect ( Section V , C ) , which is also unequivocal proof that ...
... acid or trifluoroacetic acid to the helicogenic solvent . This is shown by the fact that PBLG in 80 % ethylene dichloride- 20 % dichloroacetic acid gives a polyelectrolyte effect ( Section V , C ) , which is also unequivocal proof that ...
Page 140
... acids which are soluble in organic solvents or for those soluble in water ( e.g. , poly - L - glutamic acid ) , is promoted by electrostatic repulsion of fixed charges on the peptide groups in the former case ( Brad- bury and Fenn ...
... acids which are soluble in organic solvents or for those soluble in water ( e.g. , poly - L - glutamic acid ) , is promoted by electrostatic repulsion of fixed charges on the peptide groups in the former case ( Brad- bury and Fenn ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone