Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 386
... atom derivative for the solution of the protein structure , the locations of the heavy atoms in the unit cell are found . Once the struc- ture has been solved , the location of atoms in other isomorphous deriv- atives has proved to be a ...
... atom derivative for the solution of the protein structure , the locations of the heavy atoms in the unit cell are found . Once the struc- ture has been solved , the location of atoms in other isomorphous deriv- atives has proved to be a ...
Page 387
... atoms in side chains of the proteins . In a number of cases usable derivatives have been obtained by soaking the protein crystal in ammonium sulfate mother liquor containing K2HgI4 . In such a system any of the four species HgI , 2 ...
... atoms in side chains of the proteins . In a number of cases usable derivatives have been obtained by soaking the protein crystal in ammonium sulfate mother liquor containing K2HgI4 . In such a system any of the four species HgI , 2 ...
Page 390
... atoms . For example , grouping of iron atoms in pairs has been demonstrated by paramagnetic resonance spectros- copy of the nonheme iron proteins putidaredoxin ( Tsibris et al . , 1968 , and adrenoxin ( Orme - Johnson et al . , 1968 ) ...
... atoms . For example , grouping of iron atoms in pairs has been demonstrated by paramagnetic resonance spectros- copy of the nonheme iron proteins putidaredoxin ( Tsibris et al . , 1968 , and adrenoxin ( Orme - Johnson et al . , 1968 ) ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone