Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 86
... band center at 1 atm pressure can be calculated from the approximate relationship of Eq . ( 69 ) 62x0 Bo Po ° = Po + ... band center . Values of Bo for a range of useful gradient - forming salts and densities are given by Ifft et al ...
... band center at 1 atm pressure can be calculated from the approximate relationship of Eq . ( 69 ) 62x0 Bo Po ° = Po + ... band center . Values of Bo for a range of useful gradient - forming salts and densities are given by Ifft et al ...
Page 89
... band of macro- solute and is determined from either schlieren or absorption data . In the case of a schlieren trace ( Fig . 30 ) , the center of the band is taken as the point of intersection of the biphasic trace due to the solute band ...
... band of macro- solute and is determined from either schlieren or absorption data . In the case of a schlieren trace ( Fig . 30 ) , the center of the band is taken as the point of intersection of the biphasic trace due to the solute band ...
Page 244
... bands can be avoided by using the least - squares method ( Pitha and Jones , 1966 ; Fraser and Suzuki , 1966 , 1970a ) in which values of the band parameters for a particular model are optimized on the basis that the mean - square ...
... bands can be avoided by using the least - squares method ( Pitha and Jones , 1966 ; Fraser and Suzuki , 1966 , 1970a ) in which values of the band parameters for a particular model are optimized on the basis that the mean - square ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone