Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 185
... cell . Each small cell , too , due to unavoidable manufacturing tolerances , is slightly different . Sometimes it is a trifle out of square , for example , so that individual small cells used must be standardized separately . We have ...
... cell . Each small cell , too , due to unavoidable manufacturing tolerances , is slightly different . Sometimes it is a trifle out of square , for example , so that individual small cells used must be standardized separately . We have ...
Page 186
... cell 30 - ml cell O 4.6 O 1 4.3 1.5 3.0 6.0 10.5 15.0 30.0 60.0 120.0 Fluorescein concentration ( gm / liter x 106 ) 240.0 FIG . 11. Typical calibration data for 1 cm square cells , using fluorescein over a 160 - fold concentration ...
... cell 30 - ml cell O 4.6 O 1 4.3 1.5 3.0 6.0 10.5 15.0 30.0 60.0 120.0 Fluorescein concentration ( gm / liter x 106 ) 240.0 FIG . 11. Typical calibration data for 1 cm square cells , using fluorescein over a 160 - fold concentration ...
Page 196
... cells are generally useful to within 30 ° of the exit beam ; below this angle internal reflections become unavoidably large . This cell , in common with most light - scattering cells used in the second generation of light - scattering ...
... cells are generally useful to within 30 ° of the exit beam ; below this angle internal reflections become unavoidably large . This cell , in common with most light - scattering cells used in the second generation of light - scattering ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone