Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 5
... centrifugal field of about 250,000 g , corresponding to almost 60,000 rpm for a conventional ultracentrifuge rotor . Under this acceleration the solute protein molecules move with a steady terminal velocity , along a radial path through ...
... centrifugal field of about 250,000 g , corresponding to almost 60,000 rpm for a conventional ultracentrifuge rotor . Under this acceleration the solute protein molecules move with a steady terminal velocity , along a radial path through ...
Page 67
... centrifugal fields , the latter is less likely to be a problem in equilibrium methods than in velocity methods . The integrated forms of the sedimentation equilibrium equa- tion cannot be used profitably because , as a consequence of ...
... centrifugal fields , the latter is less likely to be a problem in equilibrium methods than in velocity methods . The integrated forms of the sedimentation equilibrium equa- tion cannot be used profitably because , as a consequence of ...
Page 94
... centrifugal force similar to the M.S.E. machine . The rotor speed is electronically controlled and is measured to an accuracy of ± 10 rpm . The rotor can be thermostated at temperatures between 0 ° and 30 ° C and rotates in a hydrogen ...
... centrifugal force similar to the M.S.E. machine . The rotor speed is electronically controlled and is measured to an accuracy of ± 10 rpm . The rotor can be thermostated at temperatures between 0 ° and 30 ° C and rotates in a hydrogen ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone