Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 100
... coil root - mean - square radius of gyration of random coil number of bonds in random coil length of each bond in random coil structural parameter related to stiffness of random coil expansion factor universal constant in Flory - Fox ...
... coil root - mean - square radius of gyration of random coil number of bonds in random coil length of each bond in random coil structural parameter related to stiffness of random coil expansion factor universal constant in Flory - Fox ...
Page 120
... coil due to interactions with the solvent . It is defined by the equation a2 = ( r2 ) av ( ro2 ) av ( 39 ) where ( ro2 ) av is the mean square end - to - end length of the so - called “ un- perturbed chain " when the excluded volume is ...
... coil due to interactions with the solvent . It is defined by the equation a2 = ( r2 ) av ( ro2 ) av ( 39 ) where ( ro2 ) av is the mean square end - to - end length of the so - called “ un- perturbed chain " when the excluded volume is ...
Page 140
... Coil Transition The reversible interconversion of helix to flexible coil was originally observed for PBLG by means of optical rotation ( Doty and Yang , 1956 ) and viscometry ( Doty , 1957 ) . Since then a tremendous amount of work has ...
... Coil Transition The reversible interconversion of helix to flexible coil was originally observed for PBLG by means of optical rotation ( Doty and Yang , 1956 ) and viscometry ( Doty , 1957 ) . Since then a tremendous amount of work has ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone