Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 31
... corresponding to this minimum and the pure solvent is thus one fringe . If the cross hairs are moved on to the next minimum , at the corresponding position in the cell the concentration is two fringes different from pure solvent . To ...
... corresponding to this minimum and the pure solvent is thus one fringe . If the cross hairs are moved on to the next minimum , at the corresponding position in the cell the concentration is two fringes different from pure solvent . To ...
Page 85
... corresponding to the band center may be defined fairly easily . However , deciding what the density is at this point , as will be seen below , is more difficult . If schlieren optics are used , the center of the band is defined by the ...
... corresponding to the band center may be defined fairly easily . However , deciding what the density is at this point , as will be seen below , is more difficult . If schlieren optics are used , the center of the band is defined by the ...
Page 89
... corresponding to the center of the band of macro- solute and is determined from either schlieren or absorption data ... corresponding to each concentration value and x is the radial distance corresponding to the band center . If the ...
... corresponding to the center of the band of macro- solute and is determined from either schlieren or absorption data ... corresponding to each concentration value and x is the radial distance corresponding to the band center . If the ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone