Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 70
... density gradient which is at all points greater than the negative density gradient due to solute at the leading edge of the zone . In the method of Rosenbloom and Schumaker ( 1963 ) a density gradient is formed in the solvent layer ...
... density gradient which is at all points greater than the negative density gradient due to solute at the leading edge of the zone . In the method of Rosenbloom and Schumaker ( 1963 ) a density gradient is formed in the solvent layer ...
Page 73
... density gradient of the protein solute is proportional to the initial protein concentration and is greatest during the first minutes of the run . After 15 minutes the maximum negative density gradient for the hypothetical protein ...
... density gradient of the protein solute is proportional to the initial protein concentration and is greatest during the first minutes of the run . After 15 minutes the maximum negative density gradient for the hypothetical protein ...
Page 84
... gradient - forming salt alone . 4. The Different Density Gradients of Importance in Density Gradient Experiments a . The Composition Density Gradient . This is due to the redistribution of salt under the influence of the centrifugal ...
... gradient - forming salt alone . 4. The Different Density Gradients of Importance in Density Gradient Experiments a . The Composition Density Gradient . This is due to the redistribution of salt under the influence of the centrifugal ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone