Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 114
... derived for very dilute solutions and nsp с = [ n ] V = v ( 27 ) M where v is the specific volume of the solute , i.e. , the volume occupied by dry solute ( 1 gm ) plus solvent which is bound to it . The partial specific volume is often ...
... derived for very dilute solutions and nsp с = [ n ] V = v ( 27 ) M where v is the specific volume of the solute , i.e. , the volume occupied by dry solute ( 1 gm ) plus solvent which is bound to it . The partial specific volume is often ...
Page 249
... derive from both phases while other bands derive from only one phase , and these methods must be used with caution . An important practical aspect of interpreting dichroism , which was neglected in most earlier work , is that with ...
... derive from both phases while other bands derive from only one phase , and these methods must be used with caution . An important practical aspect of interpreting dichroism , which was neglected in most earlier work , is that with ...
Page 312
... derived from X - ray crystallography , of a 2 - fold symmetry axis in the molecule , a model of the structure has been . derived with some confidence . The molecule is essentially flat , and four internal hydrogen bonds are defined ...
... derived from X - ray crystallography , of a 2 - fold symmetry axis in the molecule , a model of the structure has been . derived with some confidence . The molecule is essentially flat , and four internal hydrogen bonds are defined ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone