Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 36
... determination of molecular weight by the equilibrium method . The species whose molecular weight is determined is defined by the con- centration units used in the evaluation of the density increment or partial specific volume ( Section ...
... determination of molecular weight by the equilibrium method . The species whose molecular weight is determined is defined by the con- centration units used in the evaluation of the density increment or partial specific volume ( Section ...
Page 89
... determined and may be calculated as the product of the experimentally determined buoyancy density gradient ( Eq . 73 ) and the factor ( 1 − a ) . a is defined by Eq . ( 76 ) - απ дро ' da , o да1 / P dp0 ( 76 ) where ( po / a ) p is ...
... determined and may be calculated as the product of the experimentally determined buoyancy density gradient ( Eq . 73 ) and the factor ( 1 − a ) . a is defined by Eq . ( 76 ) - απ дро ' da , o да1 / P dp0 ( 76 ) where ( po / a ) p is ...
Page 125
... determined in practice by use of the Mark - Houwink equation 10 [ n ] n = KMM , w ( 50 ) in which K , and a have been determined by measurements of M , or M „ on a series of well - fractionated ( homogeneous ) samples . Alternatively ...
... determined in practice by use of the Mark - Houwink equation 10 [ n ] n = KMM , w ( 50 ) in which K , and a have been determined by measurements of M , or M „ on a series of well - fractionated ( homogeneous ) samples . Alternatively ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone