Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 30
... distances from the center of rotation , are determined as distances along the x - axis . Because rotors stretch under the influence of high centrifugal stress , the distance from the center of the rotor to the reference edge increases ...
... distances from the center of rotation , are determined as distances along the x - axis . Because rotors stretch under the influence of high centrifugal stress , the distance from the center of the rotor to the reference edge increases ...
Page 41
... distance x , from the center of rotation ; sedimentation is carried out so that the partition is always in the plateau region . After a known time of sedimentation t , the average concentration cu in the upper compartment is measured ...
... distance x , from the center of rotation ; sedimentation is carried out so that the partition is always in the plateau region . After a known time of sedimentation t , the average concentration cu in the upper compartment is measured ...
Page 86
... distance has been calculated by Ifft et al . ( 1961 ) , their data may be used to calculate xe . If the requisite data are not available , then it may be assumed that r'e is a reasonable . approximation for the isoconcentration distance ...
... distance has been calculated by Ifft et al . ( 1961 ) , their data may be used to calculate xe . If the requisite data are not available , then it may be assumed that r'e is a reasonable . approximation for the isoconcentration distance ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone