Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 11
... distribution of solute in which the concentration increases down the cell is established . The concentration distribution for a single solute is characterized by its molecular weight M1 , partial specific volume v1 , and nonideality ...
... distribution of solute in which the concentration increases down the cell is established . The concentration distribution for a single solute is characterized by its molecular weight M1 , partial specific volume v1 , and nonideality ...
Page 83
... distribution to give a distribution that is within 1 % of the equilibrium concentration over two standard deviations about the band center . Equation ( 62 ) assumes that the equilibrium salt gradient is already established at zero time ...
... distribution to give a distribution that is within 1 % of the equilibrium concentration over two standard deviations about the band center . Equation ( 62 ) assumes that the equilibrium salt gradient is already established at zero time ...
Page 89
... distribution of the macrosolute at equilibrium . With schlieren optics σ is most simply obtained by directly measuring the distances of the maximum and minimum of the biphasic pattern , in a radial direction from the band center . In ...
... distribution of the macrosolute at equilibrium . With schlieren optics σ is most simply obtained by directly measuring the distances of the maximum and minimum of the biphasic pattern , in a radial direction from the band center . In ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone