Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 55
... Equilibrium at the Menisci Only A. THEORETICAL BASIS The sedimentation equilibrium is characterized by no net flow of the solute at any point in the cell when equilibrium is established . Archibald ( 1947 ) pointed out that during the ...
... Equilibrium at the Menisci Only A. THEORETICAL BASIS The sedimentation equilibrium is characterized by no net flow of the solute at any point in the cell when equilibrium is established . Archibald ( 1947 ) pointed out that during the ...
Page 67
... Equilibrium a . The Application of Differential Equations When Interactions Exist . The equilibrium method may be used to study systems in which several components exist in solution at chemical equilibrium . However , the equi- librium ...
... Equilibrium a . The Application of Differential Equations When Interactions Exist . The equilibrium method may be used to study systems in which several components exist in solution at chemical equilibrium . However , the equi- librium ...
Page 83
... Equilibrium The approach to equilibrium involves both the establishment of sedi- mentation equilibrium for the salt which provides the density gradient , followed by the subsequent migration of the protein to its isopycnic posi- tion in ...
... Equilibrium The approach to equilibrium involves both the establishment of sedi- mentation equilibrium for the salt which provides the density gradient , followed by the subsequent migration of the protein to its isopycnic posi- tion in ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone