Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 259
... estimate integrated areas . Even then , it is necessary to assume , in the absence of contrary evidence , that the inte- grated intensity per mole residue is the same for all conformations . In addition , proper allowance must be made ...
... estimate integrated areas . Even then , it is necessary to assume , in the absence of contrary evidence , that the inte- grated intensity per mole residue is the same for all conformations . In addition , proper allowance must be made ...
Page 263
... estimate ob- tained from optical rotatory dispersion studies is excellent ( Fig . 23 ) , but when the results from ... estimates of the a / ẞ ratio it would be essential to resolve the spectrum into its component bands . To demonstrate ...
... estimate ob- tained from optical rotatory dispersion studies is excellent ( Fig . 23 ) , but when the results from ... estimates of the a / ẞ ratio it would be essential to resolve the spectrum into its component bands . To demonstrate ...
Page 315
... estimated 90 % helical content . In both studies the helicity of the PBLG samples was estimated from the CaH resonances . Markley et al . ( 1967 ) used the observed chemical shifts ( 8obs ) , of the single resonance to calculate the ...
... estimated 90 % helical content . In both studies the helicity of the PBLG samples was estimated from the CaH resonances . Markley et al . ( 1967 ) used the observed chemical shifts ( 8obs ) , of the single resonance to calculate the ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone