Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 60
... experiments are carried out on mixtures of proteins that do interact with each other , the results of those experiments must be inter- preted with extreme care since some fundamental assumptions applicable to noninteracting systems do ...
... experiments are carried out on mixtures of proteins that do interact with each other , the results of those experiments must be inter- preted with extreme care since some fundamental assumptions applicable to noninteracting systems do ...
Page 69
... experiments in which the velocity of a sedimentation boundary between a solvent and a uniform concentration solution is measured ( Fig . 2 ) . In such experiments the protein molecules sediment into a protein solution . Zonal velocity ...
... experiments in which the velocity of a sedimentation boundary between a solvent and a uniform concentration solution is measured ( Fig . 2 ) . In such experiments the protein molecules sediment into a protein solution . Zonal velocity ...
Page 174
... experiments must be carried out in order to ob- tain M2 , M4 , B22 , and B. These experiments involve ( 1 ) a mixture of the two proteins , under interacting conditions ; ( 2 ) each protein individ- ually in the same solvent . The ...
... experiments must be carried out in order to ob- tain M2 , M4 , B22 , and B. These experiments involve ( 1 ) a mixture of the two proteins , under interacting conditions ; ( 2 ) each protein individ- ually in the same solvent . The ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone