Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 70
... gradient which is at all points greater than the negative density gradient due to solute at the leading edge of the zone . In the method of Rosenbloom and Schumaker ( 1963 ) a density gradient is formed in the solvent layer before the ...
... gradient which is at all points greater than the negative density gradient due to solute at the leading edge of the zone . In the method of Rosenbloom and Schumaker ( 1963 ) a density gradient is formed in the solvent layer before the ...
Page 73
... gradient needed just to prevent convection of the protein zone ; it is higher than the gradient needed for nucleic acid zones . In the case of preformed den- sity gradients , the steepness of the gradient and the form of the gradient ...
... gradient needed just to prevent convection of the protein zone ; it is higher than the gradient needed for nucleic acid zones . In the case of preformed den- sity gradients , the steepness of the gradient and the form of the gradient ...
Page 84
... gradient- forming salt and the protein , the other the gradient - forming salt alone . 4. The Different Density Gradients of Importance in Density Gradient Experiments a . The Composition Density Gradient . This is due to the ...
... gradient- forming salt and the protein , the other the gradient - forming salt alone . 4. The Different Density Gradients of Importance in Density Gradient Experiments a . The Composition Density Gradient . This is due to the ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone