Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 184
... intensity is measured after it has passed through the cell ( and is actually a transmitted intensity ) , this reflection subtracts . intensity from the measured 0 ° beam and must be corrected for . In in- struments where the true ...
... intensity is measured after it has passed through the cell ( and is actually a transmitted intensity ) , this reflection subtracts . intensity from the measured 0 ° beam and must be corrected for . In in- struments where the true ...
Page 303
... intensity , and will lead to an under- estimate of T2 . Operational conditions are optimized to obtain undistorted absorption peaks at the maximum signal - to - noise ratio . For broad sig- nals with short T2 values , higher rf intensities ...
... intensity , and will lead to an under- estimate of T2 . Operational conditions are optimized to obtain undistorted absorption peaks at the maximum signal - to - noise ratio . For broad sig- nals with short T2 values , higher rf intensities ...
Page 419
... intensity without change in shape . The extent of the reduction of the heme iron ( III ) signal at equilibrium ( 2 or more days ) depended linearly on the mole ratio of the copper ( II ) . It was essen- tially complete at a mole ratio ...
... intensity without change in shape . The extent of the reduction of the heme iron ( III ) signal at equilibrium ( 2 or more days ) depended linearly on the mole ratio of the copper ( II ) . It was essen- tially complete at a mole ratio ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone