Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 128
... limited to rigid asymmetric particles . For further information on this subject , the reader is referred to the excellent review by Yang ( 1961a ) . E. CONCENTRATION OF SOLUTE : HUGGINS CONSTANT k ' The Huggins constant k ' ( Eq . 2 ) ...
... limited to rigid asymmetric particles . For further information on this subject , the reader is referred to the excellent review by Yang ( 1961a ) . E. CONCENTRATION OF SOLUTE : HUGGINS CONSTANT k ' The Huggins constant k ' ( Eq . 2 ) ...
Page 218
... limited to be of value . Unless there is some visual indication of the condition in which the adjustment required is out- side the range of the automatic slit control , errors may be introduced in an insidious way . c . Automatic Gain ...
... limited to be of value . Unless there is some visual indication of the condition in which the adjustment required is out- side the range of the automatic slit control , errors may be introduced in an insidious way . c . Automatic Gain ...
Page 222
... limited and there is considerable chromatic aberration at the lower end of the range . The change in speci- men beam signal due to chromatic aberration may be overcome by run- ning a second trace with no specimen in the beam condenser ...
... limited and there is considerable chromatic aberration at the lower end of the range . The change in speci- men beam signal due to chromatic aberration may be overcome by run- ning a second trace with no specimen in the beam condenser ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone