Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 38
... maximum speed may be used . For more rapidly sedimenting species , the speed is reduced in proportion to the square ... Maximum Ordinate . The position of the maximum ordinate of the schlieren peak is determined as described in Section ...
... maximum speed may be used . For more rapidly sedimenting species , the speed is reduced in proportion to the square ... Maximum Ordinate . The position of the maximum ordinate of the schlieren peak is determined as described in Section ...
Page 73
... maximum concentration ( taken from Vinograd and Bruner , 1966b ) . ( Reproduced by courtesy of the copyright owner ... maximum negative density gradient of the protein solute is proportional to the initial protein concentration and is ...
... maximum concentration ( taken from Vinograd and Bruner , 1966b ) . ( Reproduced by courtesy of the copyright owner ... maximum negative density gradient of the protein solute is proportional to the initial protein concentration and is ...
Page 94
... maximum centrifugal force similar to the M.S.E. machine . The rotor speed is electronically controlled and is measured to an accuracy of ± 10 rpm . The rotor can be thermostated at temperatures between 0 ° and 30 ° C and rotates in a ...
... maximum centrifugal force similar to the M.S.E. machine . The rotor speed is electronically controlled and is measured to an accuracy of ± 10 rpm . The rotor can be thermostated at temperatures between 0 ° and 30 ° C and rotates in a ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone