Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 93
... means of a photomultiplier which mechanically scans the image of the ultracen- trifuge cell . The logarithm of the photocurrent is plotted by means of a pen recorder . The system can be operated in a double - beam manner to eliminate ...
... means of a photomultiplier which mechanically scans the image of the ultracen- trifuge cell . The logarithm of the photocurrent is plotted by means of a pen recorder . The system can be operated in a double - beam manner to eliminate ...
Page 94
... means of an air - turbine drive which has the advantage of simplicity and freedom from vibration compared with the electric motor gearbox combination . Rotor speeds of up to 60,000 rpm are possible , giving a maximum centrifugal force ...
... means of an air - turbine drive which has the advantage of simplicity and freedom from vibration compared with the electric motor gearbox combination . Rotor speeds of up to 60,000 rpm are possible , giving a maximum centrifugal force ...
Page 180
... means of collimation or focusing to obtain a well - defined or parallel beam , a cell for containing the material under study , a collimation system for the scattered light , and some means of comparing the intensity of the incident and ...
... means of collimation or focusing to obtain a well - defined or parallel beam , a cell for containing the material under study , a collimation system for the scattered light , and some means of comparing the intensity of the incident and ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone