Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 167
... medium composed of two or more solvent components present in such ratios that protein - solvent interactions may make important contributions . This is desirable under three circumstances : ( 1 ) when the protein is insoluble in water ...
... medium composed of two or more solvent components present in such ratios that protein - solvent interactions may make important contributions . This is desirable under three circumstances : ( 1 ) when the protein is insoluble in water ...
Page 179
... medium , and is the turbidity of the me- dium . Since , as a practical matter , is often quite small , being of the order of 10- to 10-5 for a typical globular protein in aqueous solution , the light incident on the cell must be intense ...
... medium , and is the turbidity of the me- dium . Since , as a practical matter , is often quite small , being of the order of 10- to 10-5 for a typical globular protein in aqueous solution , the light incident on the cell must be intense ...
Page 231
... medium . Methacrylate , Araldite , and gelatine are among the ma- terials which have been used and detailed recipes are given by Glauert ( 1965 ) . When an embedding medium is to be used , special care must be taken to ensure that this ...
... medium . Methacrylate , Araldite , and gelatine are among the ma- terials which have been used and detailed recipes are given by Glauert ( 1965 ) . When an embedding medium is to be used , special care must be taken to ensure that this ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone