Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 129
... observed effect with increasing temperature can be either an increase in [ 7 ] ( usually observed in a poor solvent ) , no change , or a decrease in [ 7 ] ( usually observed with a good solvent ) , or the occur- rence of a flat maximum ...
... observed effect with increasing temperature can be either an increase in [ 7 ] ( usually observed in a poor solvent ) , no change , or a decrease in [ 7 ] ( usually observed with a good solvent ) , or the occur- rence of a flat maximum ...
Page 137
... observed also with globular proteins , providing that it is accompanied by an unfolding process . Of more current interest is the considerable increase in intrinsic viscosity which results from the fission of disulfide bonds in double ...
... observed also with globular proteins , providing that it is accompanied by an unfolding process . Of more current interest is the considerable increase in intrinsic viscosity which results from the fission of disulfide bonds in double ...
Page 292
... observed . Since coupling constants are independent of the applied field while chemical shifts are proportional to Ho , second - order fine structure is likely to be observed at low field strengths ( J ~ 8 ) , but spectra may become ...
... observed . Since coupling constants are independent of the applied field while chemical shifts are proportional to Ho , second - order fine structure is likely to be observed at low field strengths ( J ~ 8 ) , but spectra may become ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone