Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 133
... ( obtained from D ° or s ° ) in Eq . ( 56 ) allows the calculation of M. This method can give quite reasonable results ... obtained , which , like ẞ , is rather insensitive to axial ratio . A reasonable value of the latter is chosen , ẞ ...
... ( obtained from D ° or s ° ) in Eq . ( 56 ) allows the calculation of M. This method can give quite reasonable results ... obtained , which , like ẞ , is rather insensitive to axial ratio . A reasonable value of the latter is chosen , ẞ ...
Page 169
... obtained from sedi- mentation equilibrium and small - angle X - ray scattering data if the partial specific volume and electron density increment , respectively , are obtained using a dialyzed solution and the dialyzate as solution and ...
... obtained from sedi- mentation equilibrium and small - angle X - ray scattering data if the partial specific volume and electron density increment , respectively , are obtained using a dialyzed solution and the dialyzate as solution and ...
Page 303
... obtained in this way are sensitive to any distortion of the resonance signal and also to the intensity of the rf field used . Instrumental limits allow T , values to be measured in the range of 0.3- 10 sec . Values of T , may also be ...
... obtained in this way are sensitive to any distortion of the resonance signal and also to the intensity of the rf field used . Instrumental limits allow T , values to be measured in the range of 0.3- 10 sec . Values of T , may also be ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone