Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
From inside the book
Results 1-3 of 37
Page 226
... optical - null instrument ( Robinson , 1951 ) . Over the area of the reference beam atten- uator corresponding to 0.1 to 1.0 transmittance , the absolute ... optical density 0.10 0.05 0.5 Transmittance 1.0 226 R. D. B. FRASER AND E. SUZUKI.
... optical - null instrument ( Robinson , 1951 ) . Over the area of the reference beam atten- uator corresponding to 0.1 to 1.0 transmittance , the absolute ... optical density 0.10 0.05 0.5 Transmittance 1.0 226 R. D. B. FRASER AND E. SUZUKI.
Page 228
... Optical density for homogeneous dispersion FIG . 6. The effect of a nonuniform distribution of absorbing material in the optical path on the measured optical density ( after Jones , 1952 ) ; ≈ is the fraction of the beam obscured by ...
... Optical density for homogeneous dispersion FIG . 6. The effect of a nonuniform distribution of absorbing material in the optical path on the measured optical density ( after Jones , 1952 ) ; ≈ is the fraction of the beam obscured by ...
Page 236
... optical density as ordinate and with ordinate and abscissa expanded or contracted to give a convenient presentation . In addition , the spectral slit width and noise level should be chosen carefully and the operating conditions noted on ...
... optical density as ordinate and with ordinate and abscissa expanded or contracted to give a convenient presentation . In addition , the spectral slit width and noise level should be chosen carefully and the operating conditions noted on ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
31 other sections not shown
Other editions - View all
Common terms and phrases
absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone