Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 101
... particles empirical constants in the Fuoss equation for polyelectrolytes effective hydrodynamic volume of the solute particle translational frictional coefficients of spherical and actual particles translational diffusion coefficient ...
... particles empirical constants in the Fuoss equation for polyelectrolytes effective hydrodynamic volume of the solute particle translational frictional coefficients of spherical and actual particles translational diffusion coefficient ...
Page 132
... particle would have the same volume and axial ratio in viscosity measurements ( under shear stress ) , as it would under conditions of sedimentation or diffusion . However , this would be unlikely to be correct for a particle which is ...
... particle would have the same volume and axial ratio in viscosity measurements ( under shear stress ) , as it would under conditions of sedimentation or diffusion . However , this would be unlikely to be correct for a particle which is ...
Page 155
... particle , independent of any assumptions on mass , size , and shape . The steps in this calculation were : ( a ) evaluation of the interference between all pairs of scattering elements n and m ; ( b ) summation of these ; and ( c ) ...
... particle , independent of any assumptions on mass , size , and shape . The steps in this calculation were : ( a ) evaluation of the interference between all pairs of scattering elements n and m ; ( b ) summation of these ; and ( c ) ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone