Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 341
... peak of RNase A. The pK , values are for 32 ° C in 0.2 M deuteroacetate buffer ( Meadows et al . , 1968 ) . corrected for the presence of D2O ) are peak 1 , 6.7 ; peak 2 , 6.2 ; peak 3 , 5.8 ; peak 4 , 6.4 . In addition , a peak ...
... peak of RNase A. The pK , values are for 32 ° C in 0.2 M deuteroacetate buffer ( Meadows et al . , 1968 ) . corrected for the presence of D2O ) are peak 1 , 6.7 ; peak 2 , 6.2 ; peak 3 , 5.8 ; peak 4 , 6.4 . In addition , a peak ...
Page 343
... peak 2 and therefore His - 112 to peak 3. The anomalous chemical shift in the acid pH region of peak 4 ( Fig . 33 ) , and its broad line width in RNase A identified this peak as His - 48 which is the histidine in a " buried " and ...
... peak 2 and therefore His - 112 to peak 3. The anomalous chemical shift in the acid pH region of peak 4 ( Fig . 33 ) , and its broad line width in RNase A identified this peak as His - 48 which is the histidine in a " buried " and ...
Page 439
... peak in a thermogram . Endothermic reactions are represented by a peak extending below the basline , and exothermic reactions by a peak projecting upward . The peaks on a thermo- gram are frequently identified by the peak tips or the ...
... peak in a thermogram . Endothermic reactions are represented by a peak extending below the basline , and exothermic reactions by a peak projecting upward . The peaks on a thermo- gram are frequently identified by the peak tips or the ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone