Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 22
... photographic plate . In use , the metal line at the phase border is set at an angle to the image of the source slit . At all points in the ultracentrifuge cell where there are positive refrac- tive index gradients , the light rays ...
... photographic plate . In use , the metal line at the phase border is set at an angle to the image of the source slit . At all points in the ultracentrifuge cell where there are positive refrac- tive index gradients , the light rays ...
Page 25
... photographic film ( Fig . 13 ) . The intensity of blackening of the film at any point in the image of the cell is a measure of the light passing through the corresponding point in the cell . The quantitative use of such an optical ...
... photographic film ( Fig . 13 ) . The intensity of blackening of the film at any point in the image of the cell is a measure of the light passing through the corresponding point in the cell . The quantitative use of such an optical ...
Page 26
... photographic plate ; 8 , cell ; 9 , bromine - chlorine filter . requires a greater degree of operator sophistication than the photographic recording method . However , the method is very powerful , particularly for the study of ...
... photographic plate ; 8 , cell ; 9 , bromine - chlorine filter . requires a greater degree of operator sophistication than the photographic recording method . However , the method is very powerful , particularly for the study of ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone