Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 22
... plate on the photographic plate . In use , the metal line at the phase border is set at an angle to the image of the source slit . At all points in the ultracentrifuge cell where there are positive refrac- tive index gradients , the ...
... plate on the photographic plate . In use , the metal line at the phase border is set at an angle to the image of the source slit . At all points in the ultracentrifuge cell where there are positive refrac- tive index gradients , the ...
Page 31
... plate . The plate is aligned on the measuring microscope by the use of " reference " fringes that appear at each end of the pattern , and which are generated by inter- ference between rays of light passing through pairs of slits in the ...
... plate . The plate is aligned on the measuring microscope by the use of " reference " fringes that appear at each end of the pattern , and which are generated by inter- ference between rays of light passing through pairs of slits in the ...
Page 50
... plate . The baseline correction plate is measured at identical X positions to the equilibrium plate and the displacements of the buffer - buffer fringes relative to the reference fringes are expressed as fractions of a fringe and used ...
... plate . The baseline correction plate is measured at identical X positions to the equilibrium plate and the displacements of the buffer - buffer fringes relative to the reference fringes are expressed as fractions of a fringe and used ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone