Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 31
... position of the next minimum . The concentration difference between the radial position in the cell corresponding to this minimum and the pure solvent is thus one fringe . If the cross hairs are moved on to the next minimum , at the ...
... position of the next minimum . The concentration difference between the radial position in the cell corresponding to this minimum and the pure solvent is thus one fringe . If the cross hairs are moved on to the next minimum , at the ...
Page 38
... Position of the Sedimenting Boundary ( Fig . 9 ) a . Maximum Ordinate . The position of the maximum ordinate of the schlieren peak is determined as described in Section II , D , 4 . This proce- dure gives a reading ( in centimeters ) ...
... Position of the Sedimenting Boundary ( Fig . 9 ) a . Maximum Ordinate . The position of the maximum ordinate of the schlieren peak is determined as described in Section II , D , 4 . This proce- dure gives a reading ( in centimeters ) ...
Page 41
... position xa to the plateau region at position xp . Equation ( 24 ) is written in a form suitable for direct employment of Rayleigh interference measurements . A diagram- matic representation of a transport experiment interferogram is ...
... position xa to the plateau region at position xp . Equation ( 24 ) is written in a form suitable for direct employment of Rayleigh interference measurements . A diagram- matic representation of a transport experiment interferogram is ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone