Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 32
... procedure must be used to establish the absolute concentration ( in terms of a fringe number ) of one of the fringe minima in the pattern . Procedures for this are given in Section V , C , 2 . Once this is done , the ab- solute ...
... procedure must be used to establish the absolute concentration ( in terms of a fringe number ) of one of the fringe minima in the pattern . Procedures for this are given in Section V , C , 2 . Once this is done , the ab- solute ...
Page 112
... procedure consists of the measurement of the specific viscosity at a particular concentration , and at several different , known shear stresses , followed by extrapolation to zero shear stress . The latter is to be preferred over the ...
... procedure consists of the measurement of the specific viscosity at a particular concentration , and at several different , known shear stresses , followed by extrapolation to zero shear stress . The latter is to be preferred over the ...
Page 133
... procedure con- sists of the determination of molecular weight from viscosity and diffusion measurements , by use of ... procedure gives closer agreement with absolute values of M than is obtained by the first procedure described above ...
... procedure con- sists of the determination of molecular weight from viscosity and diffusion measurements , by use of ... procedure gives closer agreement with absolute values of M than is obtained by the first procedure described above ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone