Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 16
... solution over the solvent against which it was dialyzed . c is the total concentration ( gm / ml ) of protein ... protein is sedimented in the absence of added low molec- ular weight electrolyte , because of the strong electrostatic coupling ...
... solution over the solvent against which it was dialyzed . c is the total concentration ( gm / ml ) of protein ... protein is sedimented in the absence of added low molec- ular weight electrolyte , because of the strong electrostatic coupling ...
Page 20
... protein solution layers through the capillary on the face of the centerpiece . ( e ) Type II band - forming centerpiece ; the protein solution layers through a narrow gap between the centerpiece and the window . ( f ) Type III center ...
... protein solution layers through the capillary on the face of the centerpiece . ( e ) Type II band - forming centerpiece ; the protein solution layers through a narrow gap between the centerpiece and the window . ( f ) Type III center ...
Page 69
... solution is measured ( Fig . 2 ) . In such experiments the protein molecules sediment into a protein solution . Zonal velocity sedimentation experiments are carried out in such a way that a narrow band or zone of protein sediments into ...
... solution is measured ( Fig . 2 ) . In such experiments the protein molecules sediment into a protein solution . Zonal velocity sedimentation experiments are carried out in such a way that a narrow band or zone of protein sediments into ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone