Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 286
... proton , and generally it is the electrons associated with nuclei such as carbon , oxygen , and nitrogen , etc. , which play an important part in the shielding of nearby protons . The net effect on the protons may be either to shield or ...
... proton , and generally it is the electrons associated with nuclei such as carbon , oxygen , and nitrogen , etc. , which play an important part in the shielding of nearby protons . The net effect on the protons may be either to shield or ...
Page 304
... protons . The methylene protons of valine , leucine , isoleucine , lysine , proline , and arginine form a group at slightly lower fields , and methylenes attached to functional groups ( C = O , S — H etc. ) fall at even ( a ) Alanine ...
... protons . The methylene protons of valine , leucine , isoleucine , lysine , proline , and arginine form a group at slightly lower fields , and methylenes attached to functional groups ( C = O , S — H etc. ) fall at even ( a ) Alanine ...
Page 306
... protons under these conditions , but is a singlet at higher pH where the N - H protons exchange rapidly . This exchange and its effect on the correlation time of the solvent water protons has been investigated in detail by Sheinblatt ...
... protons under these conditions , but is a singlet at higher pH where the N - H protons exchange rapidly . This exchange and its effect on the correlation time of the solvent water protons has been investigated in detail by Sheinblatt ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone