Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 131
... ratio for prolate and oblate ellipsoids ; the relation between the axial ratio and viscosity increment v has been evaluated by Simha ( 1940 ) , and between axial ratio and F = f / fo by Perrin ( 1936 ) . Thus , it is possible to record ...
... ratio for prolate and oblate ellipsoids ; the relation between the axial ratio and viscosity increment v has been evaluated by Simha ( 1940 ) , and between axial ratio and F = f / fo by Perrin ( 1936 ) . Thus , it is possible to record ...
Page 132
... ratio p ; indeed , this is the ellipsoid which is equivalent to the real particle . ( 3 ) The ẞ and the 8 functions are rather insensitive to axial ratio . This is because the dependence on p of each of the two hydrodynamic quantities ...
... ratio p ; indeed , this is the ellipsoid which is equivalent to the real particle . ( 3 ) The ẞ and the 8 functions are rather insensitive to axial ratio . This is because the dependence on p of each of the two hydrodynamic quantities ...
Page 249
... ratio 0.5 0.4 0.3 0.2 11 π Dichroism 10 9 8 Dichroic ratio A 10 5 4 3 2 ( a ) 0.5 1.0 Minimum fraction of oriented chains ( b ) 0.5 Minimum fraction of oriented chains FIG . 16. The relation between dichroic ratio and the minimum value ...
... ratio 0.5 0.4 0.3 0.2 11 π Dichroism 10 9 8 Dichroic ratio A 10 5 4 3 2 ( a ) 0.5 1.0 Minimum fraction of oriented chains ( b ) 0.5 Minimum fraction of oriented chains FIG . 16. The relation between dichroic ratio and the minimum value ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone