Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 30
... reference edge increases at high speeds of rotation . For the most precise work this must be taken into account . For example , at 59,780 rpm in a Duralumin rotor , a reference . edge in a counterbalance is 0.03 cm further from the ...
... reference edge increases at high speeds of rotation . For the most precise work this must be taken into account . For example , at 59,780 rpm in a Duralumin rotor , a reference . edge in a counterbalance is 0.03 cm further from the ...
Page 38
... reference to the same arbitrary zero . The meniscus position should not change with time and if it does , a cell ... reference edges , respectively . G is the horizontal magnification factor of the ultra- centrifuge optical system and E ...
... reference to the same arbitrary zero . The meniscus position should not change with time and if it does , a cell ... reference edges , respectively . G is the horizontal magnification factor of the ultra- centrifuge optical system and E ...
Page 218
... reference beam , the reference signal is attenuated whenever a region of solvent absorption is scanned . Unless steps are taken to restore the servosignal per unit comb displace- ment to its optimum value , the photometer response will ...
... reference beam , the reference signal is attenuated whenever a region of solvent absorption is scanned . Unless steps are taken to restore the servosignal per unit comb displace- ment to its optimum value , the photometer response will ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone