Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 8
... region shown in Fig . 2 . The integration of the differential equation to describe the shape of a sedimenting boundary , that is , the zone between the zero concentration region and the plateau region , has been carried out by Fujita ...
... region shown in Fig . 2 . The integration of the differential equation to describe the shape of a sedimenting boundary , that is , the zone between the zero concentration region and the plateau region , has been carried out by Fujita ...
Page 217
... region , a calcium fluoride prism in the 1600 cm - 1 region , and sodium chloride , potassium bromide , cesium bromide , and cesium . iodide prisms for the lower frequency ranges . Earlier studies of overtone and combination bands in ...
... region , a calcium fluoride prism in the 1600 cm - 1 region , and sodium chloride , potassium bromide , cesium bromide , and cesium . iodide prisms for the lower frequency ranges . Earlier studies of overtone and combination bands in ...
Page 351
... region with progressive denaturation by urea were mainly due to decreased shielding of Phe , Tyr , and Trp , which indicated the presence of a highly compact aromatic region in the native enzyme as described for the structure determined ...
... region with progressive denaturation by urea were mainly due to decreased shielding of Phe , Tyr , and Trp , which indicated the presence of a highly compact aromatic region in the native enzyme as described for the structure determined ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone