Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 334
... relative concentrations of a - NAG to B - NAG vary . Analysis of these data showed that there was very little difference in ... relatively small downfield shift which is pH independent , strongly suggesting that there is no significant ...
... relative concentrations of a - NAG to B - NAG vary . Analysis of these data showed that there was very little difference in ... relatively small downfield shift which is pH independent , strongly suggesting that there is no significant ...
Page 367
... relatively difficult , partly because of lack of wide experience with peptide systems ( Gurd , 1962 ; Hartzell and ... relatively concisely developed because the ideas presented are rein- forced elsewhere in the presentation . Section ...
... relatively difficult , partly because of lack of wide experience with peptide systems ( Gurd , 1962 ; Hartzell and ... relatively concisely developed because the ideas presented are rein- forced elsewhere in the presentation . Section ...
Page 409
... relatively closer to that of a unimolecular transi- tion state than to that of a conventional , stable molecule . This configura- tion can be thought of , in a sense , as an energetically unstable , sometimes expressed as a " poised ...
... relatively closer to that of a unimolecular transi- tion state than to that of a conventional , stable molecule . This configura- tion can be thought of , in a sense , as an energetically unstable , sometimes expressed as a " poised ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone