Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 117
... rod ( Doty et al . , 1956 ) . The evaluation of the dimensions of solvated rods is treated in Section VI , A , and the ques- tion of rigidity of rods is considered in Section VI , E , 1 . The intrinsic viscosity of a model which ...
... rod ( Doty et al . , 1956 ) . The evaluation of the dimensions of solvated rods is treated in Section VI , A , and the ques- tion of rigidity of rods is considered in Section VI , E , 1 . The intrinsic viscosity of a model which ...
Page 123
... Rods At the other extreme there is the case of rigid rods which can be ap- proximated by prolate ellipsoids of revolution . For large values of the axial ratio p , it is seen in Fig . 2 that the viscosity increment v is propor- tional ...
... Rods At the other extreme there is the case of rigid rods which can be ap- proximated by prolate ellipsoids of revolution . For large values of the axial ratio p , it is seen in Fig . 2 that the viscosity increment v is propor- tional ...
Page 128
... rods such as helical PBLG , to determine their lengths and rotational diffusion coefficients by measure- ment of [ 7 ] as a function of shearing stress . The results agree satisfac- torily ( Yang , 1959 , 1961a ) with those obtained by ...
... rods such as helical PBLG , to determine their lengths and rotational diffusion coefficients by measure- ment of [ 7 ] as a function of shearing stress . The results agree satisfac- torily ( Yang , 1959 , 1961a ) with those obtained by ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone