Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 149
... scattering of light that we owe the glowing tones that we admire in many a sunset . It was probably Helmholtz who first spoke of light scattering in relation to biology when he attributed the magnetism of a pair of blue eyes to nothing ...
... scattering of light that we owe the glowing tones that we admire in many a sunset . It was probably Helmholtz who first spoke of light scattering in relation to biology when he attributed the magnetism of a pair of blue eyes to nothing ...
Page 185
... scattering or increment of scattering , I.16 The excess scattering of the same solution is then measured in the small cell and the " standardization factor " k is obtained k = I abs . cell I small cell ( 37 ) The proper collimating ...
... scattering or increment of scattering , I.16 The excess scattering of the same solution is then measured in the small cell and the " standardization factor " k is obtained k = I abs . cell I small cell ( 37 ) The proper collimating ...
Page 489
... scattering data , 183 , 204 light - scattering and , 153 measurement of , 162-166 , 198-200 Refractometers , differential , 198-200 , 208 Ribonuclease abnormal tyrosines in , 339-340 copper binding to , 413 denaturation of , 337-340 ...
... scattering data , 183 , 204 light - scattering and , 153 measurement of , 162-166 , 198-200 Refractometers , differential , 198-200 , 208 Ribonuclease abnormal tyrosines in , 339-340 copper binding to , 413 denaturation of , 337-340 ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone