Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 51
... shown in Fig . 17. If the fringe chosen runs off the pattern , it is only necessary to transfer to the next fringe as shown . It is always worth- while obtaining a baseline correction photograph with the high speed method because of the ...
... shown in Fig . 17. If the fringe chosen runs off the pattern , it is only necessary to transfer to the next fringe as shown . It is always worth- while obtaining a baseline correction photograph with the high speed method because of the ...
Page 138
... shown that PBLG had the dimensions of a rigid rod with a length of 1.5 A per residue and a diameter of 14.9 A when dissolved in N , N ' - formdimethylamide , or in chloroform saturated with formamide . The rigid - rod model applied for ...
... shown that PBLG had the dimensions of a rigid rod with a length of 1.5 A per residue and a diameter of 14.9 A when dissolved in N , N ' - formdimethylamide , or in chloroform saturated with formamide . The rigid - rod model applied for ...
Page 152
... shown in Fig . 2a . Over a period of time , the total polarizability of the volume element will vary as shown in Fig . 2b . The fluctuation theory of light scattering has its origin in the works of Smoluchowski ( 1908 ) , Einstein ...
... shown in Fig . 2a . Over a period of time , the total polarizability of the volume element will vary as shown in Fig . 2b . The fluctuation theory of light scattering has its origin in the works of Smoluchowski ( 1908 ) , Einstein ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone