Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 11
... single solvent component . This will not be a valid assumption if one of the low molecular weight solutes binds preferentially to the protein , or if the low molecular weight solutes each distribute very differently down the cell during ...
... single solvent component . This will not be a valid assumption if one of the low molecular weight solutes binds preferentially to the protein , or if the low molecular weight solutes each distribute very differently down the cell during ...
Page 42
... single sharp peak does not indicate necessarily a single homogeneous protein . Broadly speaking the possibilities are : ( a ) a single protein , ( b ) two proteins with very similar sedimentation coefficients , ( c ) a major protein ...
... single sharp peak does not indicate necessarily a single homogeneous protein . Broadly speaking the possibilities are : ( a ) a single protein , ( b ) two proteins with very similar sedimentation coefficients , ( c ) a major protein ...
Page 63
... single equilibrium to form dimers , only a single asymmetric schlieren peak is seen . If the sys- tem is examined over a range of concentrations , the asymmetry may de- crease as concentration is increased . In the case of a solute in ...
... single equilibrium to form dimers , only a single asymmetric schlieren peak is seen . If the sys- tem is examined over a range of concentrations , the asymmetry may de- crease as concentration is increased . In the case of a solute in ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone