Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 16
... solution over the solvent against which it was dialyzed . c is the total concentration ( gm / ml ) of protein ... solution . According to the classical theory ( Pedersen , 1958 ) the smaller the proportion of the elec- trical ...
... solution over the solvent against which it was dialyzed . c is the total concentration ( gm / ml ) of protein ... solution . According to the classical theory ( Pedersen , 1958 ) the smaller the proportion of the elec- trical ...
Page 31
... solution or solvent , the so - called " air " fringes produced by this region can be very helpful in aligning the imaginary line joining the reference fringes parallel to the x - axis of the microscope . The Rayleigh system essentially ...
... solution or solvent , the so - called " air " fringes produced by this region can be very helpful in aligning the imaginary line joining the reference fringes parallel to the x - axis of the microscope . The Rayleigh system essentially ...
Page 84
... solution at sedi- mentation equilibrium does not bring about redistribution of the solute but does result in compression of the solution . Equation ( 65 ) thus includes the isothermal compressibility of the solution K ( dp / dx ) phys ...
... solution at sedi- mentation equilibrium does not bring about redistribution of the solute but does result in compression of the solution . Equation ( 65 ) thus includes the isothermal compressibility of the solution K ( dp / dx ) phys ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone