Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 31
... solvent , the so - called " air " fringes produced by this region can be very helpful in aligning the imaginary line joining the reference fringes parallel to the x - axis of the microscope . The Rayleigh system essentially allows ...
... solvent , the so - called " air " fringes produced by this region can be very helpful in aligning the imaginary line joining the reference fringes parallel to the x - axis of the microscope . The Rayleigh system essentially allows ...
Page 161
... solvent components are odd numbered , 3 , 5 , etc. The general light - scattering equation for a system of v ... solvent , Ar is the excess turbidity of the solution over that of solvent , m , is the molality of component i ( moles per ...
... solvent components are odd numbered , 3 , 5 , etc. The general light - scattering equation for a system of v ... solvent , Ar is the excess turbidity of the solution over that of solvent , m , is the molality of component i ( moles per ...
Page 189
... solvent trapped in the pores of the disk . This layers on top of the denser protein solution and , at the end of the filtration it should be mixed in by gentle swirling to give a uniform concentration throughout . Solvent is cleaned by ...
... solvent trapped in the pores of the disk . This layers on top of the denser protein solution and , at the end of the filtration it should be mixed in by gentle swirling to give a uniform concentration throughout . Solvent is cleaned by ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone