Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 36
... SPECIFIC VOLUME OF THE SOLUTE Partial specific volumes are needed for the determination of the molec- ular weight by means of the classical equations . The Casassa and Eisen- berg treatment of multicomponent systems described in Section ...
... SPECIFIC VOLUME OF THE SOLUTE Partial specific volumes are needed for the determination of the molec- ular weight by means of the classical equations . The Casassa and Eisen- berg treatment of multicomponent systems described in Section ...
Page 79
... specific volume ( Un ) by means of the relation Uh = ( 1 / po ) ( 55 ) In general the hydrodynamic partial specific volume is not identical to the partial specific volume determined by conventional means ( see Part C of this series ) ...
... specific volume ( Un ) by means of the relation Uh = ( 1 / po ) ( 55 ) In general the hydrodynamic partial specific volume is not identical to the partial specific volume determined by conventional means ( see Part C of this series ) ...
Page 169
... specific volume using the dialyzate as reference solvent , ø ' , is related to the apparent partial specific volume measured in the usual way , ø , by φ ' = მყვ $ - ( 393 ) T ... ( ~ - ( Va ) T.p.m . ) - ag T.P.43 Pa where p . is the ...
... specific volume using the dialyzate as reference solvent , ø ' , is related to the apparent partial specific volume measured in the usual way , ø , by φ ' = მყვ $ - ( 393 ) T ... ( ~ - ( Va ) T.p.m . ) - ag T.P.43 Pa where p . is the ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone