Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
From inside the book
Results 1-3 of 48
Page 48
... speed from Eq . ( 33 ) . = For the high speed method , Yphantis ( 1964 ) recommends that cb / Ca 5 so that once again if M is known approximately , the speed may be cal- culated . Following Van Holde ( 1967 ) , graphs of speed against ...
... speed from Eq . ( 33 ) . = For the high speed method , Yphantis ( 1964 ) recommends that cb / Ca 5 so that once again if M is known approximately , the speed may be cal- culated . Following Van Holde ( 1967 ) , graphs of speed against ...
Page 94
... speeds and centrifugal forces are identical , and the minimum speed is 6000 rpm . The rotor operates in a vacuum at a pressure of 1 μ of mercury and at temperatures up to 150 ° C , controlled to 0.2 ° C . The rotor temperature is ...
... speeds and centrifugal forces are identical , and the minimum speed is 6000 rpm . The rotor operates in a vacuum at a pressure of 1 μ of mercury and at temperatures up to 150 ° C , controlled to 0.2 ° C . The rotor temperature is ...
Page 219
... Speed Control . The speed at which a spectrum may be scanned without introducing errors is limited by electrical damping and mechanical inertia in the photometer and recording units and by the maxi- mum rate of change of transmittance ...
... Speed Control . The speed at which a spectrum may be scanned without introducing errors is limited by electrical damping and mechanical inertia in the photometer and recording units and by the maxi- mum rate of change of transmittance ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
31 other sections not shown
Other editions - View all
Common terms and phrases
absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone