Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 149
... techniques , today light scatter- ing and small - angle X - ray scattering are recognized as powerful tools for the determination of molecular weights and structures of macromolecules , as well as for the characterization of the ...
... techniques , today light scatter- ing and small - angle X - ray scattering are recognized as powerful tools for the determination of molecular weights and structures of macromolecules , as well as for the characterization of the ...
Page 181
... techniques , particularly in the smaller sizes , and the presence of unavoidable striae requires that each individual cell be cali- brated in the instrument ( Zimm , 1948a ) and its imperfections must be corrected for . This technique ...
... techniques , particularly in the smaller sizes , and the presence of unavoidable striae requires that each individual cell be cali- brated in the instrument ( Zimm , 1948a ) and its imperfections must be corrected for . This technique ...
Page 424
... techniques ( Meadows and Jardetzky , 1968 ; Joyce and Cohn , 1969 ; Raftery et al . , 1969 ; McDonald and Phillips , 1970 ; Ling and Cope , 1969 ) should become a standard technique . The application of EPR techniques to protein ...
... techniques ( Meadows and Jardetzky , 1968 ; Joyce and Cohn , 1969 ; Raftery et al . , 1969 ; McDonald and Phillips , 1970 ; Ling and Cope , 1969 ) should become a standard technique . The application of EPR techniques to protein ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone