Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 106
... viscometer . It is nor- mal practice to evaluate K ' by measurement of the flow time t of a pure liquid of known viscosity and density . The viscometer is then calibrated for the measurement , for unknown Newtonian liquids , of / p ...
... viscometer . It is nor- mal practice to evaluate K ' by measurement of the flow time t of a pure liquid of known viscosity and density . The viscometer is then calibrated for the measurement , for unknown Newtonian liquids , of / p ...
Page 108
... viscometer ( Wada , 1954 ; Fujishige et al . , 1963 ) . Another requires the use of a multigradient viscometer which consists of three or more bulbs above a long capillary ( Holtzer et al . , 1954 ; Schurz , 1954 ) . A third procedure ...
... viscometer ( Wada , 1954 ; Fujishige et al . , 1963 ) . Another requires the use of a multigradient viscometer which consists of three or more bulbs above a long capillary ( Holtzer et al . , 1954 ; Schurz , 1954 ) . A third procedure ...
Page 111
... viscometer which consisted of a rotating outer cup and an inner cylinder of slightly smaller radius which was supported by a torsion wire . Subsequently , many different designs have been prepared in which one or other of the cylinders ...
... viscometer which consisted of a rotating outer cup and an inner cylinder of slightly smaller radius which was supported by a torsion wire . Subsequently , many different designs have been prepared in which one or other of the cylinders ...
Contents
Ultracentrifugal Analysis | 10 |
Light Scattering | 12 |
Osmotic Pressure | 13 |
Copyright | |
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absorption acid angle axial ratio axis band beam binding Biol Bradbury Brice calculated capillary centrifugal chain Chem chemical shifts column complex component concentration dependence constant copper(II denaturation density gradient determined diffusion dilution Doty effect ellipsoid equation experiments extrapolation filters Fraser frequency fringe groups Gurd Holtzer hydrogen increment instrument interaction intrinsic viscosity Kirkwood length light scattering light-scattering linear macromolecule maximum measured meniscus method molecular weight molecule Natl observed obtained optical density optical system parameters partial specific volume particle peak photographic Phys plate plateau plot Polymer Sci Proc procedure protein solution protons radius random coil Rayleigh reference refractive index refractive index increment residues resonance rotation rotor sample Schachman schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress shown in Fig slit solvent spectra spectrum speed structure synthetic boundary Tanford technique temperature Timasheff tion transmittance tube ultracentrifuge values Vinograd viscometer zero Zimm zone