Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 82
Page 1-90
... hybridization of minipreparations of plas- mid DNA . Three types of solid supports may be used for in situ hybridization of lysed bacterial colonies - nitrocellulose filters , nylon membranes , and Whatman 541 filter papers . Nylon ...
... hybridization of minipreparations of plas- mid DNA . Three types of solid supports may be used for in situ hybridization of lysed bacterial colonies - nitrocellulose filters , nylon membranes , and Whatman 541 filter papers . Nylon ...
Page 7-37
... hybridization with radiolabeled DNA or RNA followed by autoradiography . • Dot and slot hybridization , in which an excess of radiolabeled probe is hybridized to RNA that has been immobilized on a solid support ( Kafatos et al . 1979 ...
... hybridization with radiolabeled DNA or RNA followed by autoradiography . • Dot and slot hybridization , in which an excess of radiolabeled probe is hybridized to RNA that has been immobilized on a solid support ( Kafatos et al . 1979 ...
Page 7-75
... hybridization buffer . The hydrated RNA goes into solution quickly and easily and gives extremely reproducible results . Usually , 1 × 105 to 5 x 105 cpm of probe are used per hybridization . A single in vitro transcription reaction ...
... hybridization buffer . The hydrated RNA goes into solution quickly and easily and gives extremely reproducible results . Usually , 1 × 105 to 5 x 105 cpm of probe are used per hybridization . A single in vitro transcription reaction ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml