Physical Principles and Techniques of Protein Chemistry, Part 3Sydney J. Leach |
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Page 188
... buffer is by no means sufficient ( as is sometimes thought ) , since for small - angle scat- tering the difference in electron densities is decisive . In every case , it is best to dialyze the protein solution against the buffer used as ...
... buffer is by no means sufficient ( as is sometimes thought ) , since for small - angle scat- tering the difference in electron densities is decisive . In every case , it is best to dialyze the protein solution against the buffer used as ...
Page 274
... Buffer The buffer or other supporting medium should be such that there is minimal interaction with Mn2 + . The metal ion is normally at a concen- tration of the order of 10 M and as the buffer concentration is expected to be greater ...
... Buffer The buffer or other supporting medium should be such that there is minimal interaction with Mn2 + . The metal ion is normally at a concen- tration of the order of 10 M and as the buffer concentration is expected to be greater ...
Page 276
... Buffer system 1.600 2.256 0.44 Buffer system + MnCl , ( 1.2 × 10-4 M ) 0.520 Buffer system + MnCl2 + PEPCK 0.252 0.733 1.36 0.92 0.355 2.82 2.38 2.59 ( 0.71 X 10-4 M ) Buffer system + MnCl2 + PEPCK 0.150 0.212 4.72 4.28 4.65 ( 1.48 × 10 ...
... Buffer system 1.600 2.256 0.44 Buffer system + MnCl , ( 1.2 × 10-4 M ) 0.520 Buffer system + MnCl2 + PEPCK 0.252 0.733 1.36 0.92 0.355 2.82 2.38 2.59 ( 0.71 X 10-4 M ) Buffer system + MnCl2 + PEPCK 0.150 0.212 4.72 4.28 4.65 ( 1.48 × 10 ...
Contents
F Density and Composition | 16 |
Direct Volume Change | 22 |
Density | 32 |
Copyright | |
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a-helix absorption acid Amer angle B-structure band Biochem Biochemistry Biol c₂ calculated CD spectra Chem chemical potential chromophore coefficient conformation constant Cotton effects cross-section denaturation determined dialyzate difference diffusible components dilute Donnan electron density enzyme equation equilibrated equilibrium exciton experimental function gm/ml grams helical helix hemocyanin hemoglobin intensity interaction Kratky Kupke Linderstrøm-Lang macromolecules magnetic measurements membrane method mixture molal molecular weight molecule monomer myoglobin negative nondiffusible components nonideality number of moles obtained optical activity osmometers osmotic pressure parameter partial specific volume particles peptide Phys Pilz plot polymer polypeptides positive potential procedure protein component protein concentration protein solution radius of gyration random coil residues rotational strength salt sample Scatchard Schellman Section shape small-angle scattering solvent solvent medium spectrum sphere structure subunits temperature Tinoco tion transition v₂ volume change wavelength zero